Journal: BMC Veterinary Research

Article Title: Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses

doi: 10.1186/s12917-021-03126-y

Figure Lengend Snippet: Percentages (%) of cells positive for specific molecular markers by flow cytometry

Article Snippet: After incubation, the antibodies against CD11a/CD18, CD44, MHC class I, and MHC class II were coupled with secondary got antibodies conjugated to fluorescein isothiocyanate (FITC; FITC anti-mouse IgG, Rockland lmmunologicals).

Techniques:

Journal: Cytotechnology

Article Title: Sequential cultivation of human epidermal keratinocytes and dermal mesenchymal like stromal cells in vitro

doi: 10.1007/s10616-015-9857-x

Figure Lengend Snippet: Represents the list of antibodies used to characterize the HSKs and DMSCs

Article Snippet: CD-44 , Anti-human CD44 , 1:50 , BD Pharmingen (550989) , Immunophenotyping by flow cytometry.

Techniques: Transduction, Cell Culture, In Situ, Staining, Flow Cytometry, Immunohistochemistry, Immunofluorescence, Western Blot

Journal: Cytotechnology

Article Title: Sequential cultivation of human epidermal keratinocytes and dermal mesenchymal like stromal cells in vitro

doi: 10.1007/s10616-015-9857-x

Figure Lengend Snippet: Immunophenotyping of the HEKs: flow cytometry revealed the positive expression of these ketatinocyte markers such as (a, b) E-cadherin along with its specific isotype control and (c, d) keratin-5 with isotype control. Expansion and characterization of dermal-derived mesenchymal-like stromal cells (DMSCs): e Phase contrast photographs of DMSCs at early passage (P5); f late passages (P15), respectively. Immunofluorescence analysis of DMSCs using: g–i anti-vimentin; j–l anti-CD44; m–o anti-CD73 antibodies. Vimentin being a cytoskeletal protein shows cytoskeletal localization while CD44 and CD73 being cell surface antigens demonstrate surface localization. DAPI was used as a nuclear stain. Scale bars 100 μm (e–o). (Color figure online)

Article Snippet: CD-44 , Anti-human CD44 , 1:50 , BD Pharmingen (550989) , Immunophenotyping by flow cytometry.

Techniques: Flow Cytometry, Expressing, Derivative Assay, Immunofluorescence, Staining